我们的网站不完全支持您的浏览器。

We've detected that you are using an older version of Internet Explorer. Your commerce experience may be limited. Please update your browser to Internet Explorer 11 or above.

我们相信本网站可能会为您提供最好的服务:

美国

美国

语言:英语

Promega's Cookie Policy

Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.

法医DNA分析:毛细管电泳

Promega为通过毛细管电泳(CE)分析法医DNA样本提供完整的解决方案。这些包括CE分析工作流程中所有步骤的试剂和试剂盒。

产品包括在直接扩增数据库样本之前,用于拭子和卡片打孔的预处理试剂,以及用于个案样本的DNA分离系统。我们还提供PowerQuant®系统用于精确的人类特异性DNA定量,以及PowerPlex®系统用于Y特异性或常染色体DNA的STR扩增,以满足全球和区域数据库的要求。对于样品分析,CE仪器的光谱系列提供了低通量和高通量样品处理选项。

CE分析基础

The vast majority of the human genome is identical among diverse individuals. However, there are small regions of variation, especially in areas with repetitive sequences. One example is short tandem repeats (STRs) that can vary in length among individuals, with each repeat consisting of three to five nucleotides of DNA. Analysis of these repeats reveals a characteristic pattern for each individual that is inherited from paternal and maternal DNA. Forensic DNA databases use these patterns of STRs for human identification, and each national database has requirements for specific STR loci that are included.

为了描述这些STR模式或DNA图谱的特征,人类基因组DNA用聚合酶链反应(PCR)扩增,每个STR都有一套引物。引物用荧光染料标记,扩增产物用毛细管电泳按大小分离。CE仪器的荧光成像结果是每个染料通道都有一组峰,这些峰共同构成一个电泳图,作为该个体的DNA“轮廓”。对DNA图谱进行统计分析并与参考数据库进行比较,可以预测个体是否与已知的DNA图谱相匹配,具有很高的辨别能力。